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⭐ Selected Publications

2026

  • Sopalda, S., Vergara, R., Muñoz, M., Aguirre, C., Muñoz, C., & Prieto, H. (2026). Dual-gRNA CRISPR/Cas9 Deletion of CsDMR6 in Sweet Orange Supported by Improved In Vitro Regeneration. Plants, 15(17), 2664. https://doi.org/10.3390/plants15172664

  • Grbich, M., Muñoz, M., Zúñiga, G. E., Valdovinos, G., Acha, G., Vergara, R., Mora, R., Olivares, F., Olmedo, B., & Prieto, H. (2026). Transgene-Free Editing of PPO2 in Elite Potato Cultivar YAGANA for Reduced Postharvest Browning. Agronomy, 16(2), 216. https://doi.org/10.3390/agronomy16020216

  • Prieto H and Castro Á (2026) Editorial: Harnessing the power of small RNAs: advances and applications in plant gene silencing and stress response. Front. Plant Sci. 17:1924418. doi: 10.3389/fpls.2026.1924418

2025

  • Barrera M., Olmedo B., Narváez M., Moenne‑Locoz F., Rubio A., Pérez C., Cordero K., Prieto H. (2025). Protoplast-Based Regeneration Enables CRISPR/Cas9 Application in Two Temperate Japonica Rice Cultivars. Plants 14, 2059 — DOI: https://doi.org/10.3390/plants14132059 

  • Toro C., Barrera M., Olmedo B., Vergara R., Muñoz M., Olivares F., Prieto H. (2025). Agrobacterium-Mediated Transformation of Prunus persica (Peach) Immature Cotyledons. Methods in Molecular Biology 2911: 189–198 — DOI: https://doi.org/10.1007/978-1-0716-4450-8_18

  • Gaete-Loyola J., Olivares F., Saavedra G., Zúñiga T., Mora R., Ríos I., Valdovinos G., Barrera M., Almeida A., Prieto H. (2025). Artificial Sweet Cherry miRNA 396 Promotes Early Flowering in Vernalization-Dependent Arabidopsis Edi‑0 Ecotype. Plants 14, 899 — DOI: https://doi.org/10.3390/plants14060899

2024

  • Barrera M., Olmedo B., Zúñiga C., Cepeda M., Olivares F., Vergara R., Cordero K., Prieto H. (2024). Somatic Embryogenesis and Agrobacterium-Mediated Gene Transfer Procedures in Chilean Temperate Japonica Rice Varieties for Precision Breeding. Plants 13, 416 — DOI: https://doi.org/10.3390/plants13030416

2023

  • Ortiz R., Eriksson D., Visser R., Vives Vallés J., Prieto H. (2023). Editorial: Leeway to Operate With Plant Genetic Resources. Frontiers in Plant Science — DOI: https://doi.org/10.3389/fpls.2023.1249563 

  • Orbovic V., Prieto H. (2023). Editorial: New Developments in Agrobacterium-Mediated Transformation of Tree Fruit Crops, Volume II. Frontiers in Plant Science 14 — DOI: https://doi.org/10.3389/fpls.2023.1249563

2022

  • Miccoli C., Gambacorta G., Urbinati G., Santiago-Reyes M., Gentile A., Monticelli S., Caboni E., Prieto H., Verde I., Decroocq V., Vendramin E., Micali S. (2022). Novel Breeding Strategies for Tackling Present and Future Challenges in Prunus Species: The Case of Peach. Acta Horticulturae — DOI: https://doi.org/10.17660/ActaHortic.2022.1352.58

  • Soto E., Sanchez E., Nuñez C., Montes C., Rothkegel K., Andrade P., Prieto H., Almeida A. (2022). Small RNA Differential Expression Analysis Reveals miRNAs Involved in Dormancy Progression in Sweet Cherry Floral Buds. Plants 11, 2396 — DOI: https://doi.org/10.3390/plants11182396

2021

  • Olivares F., Loyola R., Olmedo B., Miccono M., Aguirre C., Vergara R., Riquelme D., Madrid G., Plantat P., Mora R., Espinoza D., Prieto H. (2021). CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons. Frontiers in Plant Science — DOI: https://doi.org/10.3389/fpls.2021.791030

  • Acha G., Vergara R., Muñoz M., Mora R., Aguirre C., Muñoz M., Kalazich J., Prieto H. (2021). A Traceable DNA-Replicon Derived Vector to Speed Up Gene Editing in Potato: Interrupting Genes Related to Undesirable Postharvest Tuber Traits as an Example. Plants 10, 1882 — DOI: https://doi.org/10.3390/plants10091882

  • Vergara R., Olivares F., Olmedo B., Toro C., Muñoz M., Zúñiga C., Mora R., Plantat P., Miccono M., Loyola R., Aguirre C., Prieto H. (2021). Gene Editing in Prunus spp.: The Challenge of Adapting Regular Gene Transfer Procedures for Precision Breeding. IntechOpen — DOI: https://doi.org/10.5772/intechopen.98843

2020

  • Medina L., Castillo C., Liempi A., Guerrero-Muñoz J., Rojas-Pirela M., Maya J.D., Prieto H., Kemmerling U. (2020). Trypanosoma cruzi and Toxoplasma gondii Induce a Differential MicroRNA Profile in Human Placental Explants. Frontiers in Immunology — DOI: https://doi.org/10.3389/fimmu.2020.595250

  • Eriksson D., Ortiz R., Visser R., Vives-Vallés J., Prieto H. (2020). Editorial: Leeway to Operate With Plant Genetic Resources. Frontiers in Plant Science — DOI: https://doi.org/10.3389/fpls.2020.00911

  • Ricci A., Sabbadini S., Prieto H., Padilla I., Dardick C., Li Z., Scorza R., Limera C., Mezzetti B., Perez-Jimenez M., Burgos L., Petri C. (2020). Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants 9, 971 — DOI: https://doi.org/10.3390/plants9080971

  • Núñez R., Aguirre C., Soto J., Salinas P., Prieto H., Paneque M. (2020). Physicochemical Parameters Affecting the Distribution and Diversity of the Water Column Microbial Community in High-Altitude Andean Lakes. Microorganisms 8, 1181 — DOI: https://doi.org/10.3390/microorganisms8081181

  • Marini D., Maeso D., Bustamante A., Prieto H. (2020). State of the Art of the Regional Platform for Early Detection of Quarantine Diseases in Fruit Crops in Latin America and the Caribbean. FONTAGRO — Available at: https://www.fontagro.org/new/proyectos/sanidad-frutales/es

2019

  • Eriksson D., Kershen D., Nepomuceno A., Pogson B.J., Prieto H., Purnhagen K., Smyth S., Wesseler J., Whelan A. (2019). A Comparison of the EU Regulatory Approach to Directed Mutagenesis with That of Other Jurisdictions. New Phytologist — DOI: https://doi.org/10.1111/nph.15627

  • Aranzana M.J., Decroocq V., Dirlewanger E., Eduardo I., Gao Z.S., Gasic K., Iezzoni A., Jung S., Peace C., Prieto H., Tao R., Verde I., Abbott A.G., Arús P. (2019). Prunus Genetics and Applications After de novo Genome Sequencing: Achievements and Prospects. Horticulture Research 6:58 — DOI: https://doi.org/10.1038/s41438-019-0140-8

  • Guo-qing S., Prieto H., Orbovic V. (2019). Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges. Frontiers in Plant Science — DOI: https://doi.org/10.3389/fpls.2019.00226

  • Rubio J., Sánchez E., Tricon D., et al. (2019). Silencing of eIFiso4G in Japanese Plum Impacts Susceptibility to Plum Pox Virus and Small RNA Production. BMC Plant Biology 19, 440 — DOI: https://doi.org/10.1186/s12870-019-2047-9 (doi.org in Bing)

  • Prieto H., Miccono M., Aguirre C., Sánchez E., Castro Á. (2019). Grape Biotechnology: Past, Present, and Future. The Grape Genome (Springer) — DOI: https://doi.org/10.1007/978-3-030-18601-2_16

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