WHO WE ARE
​
Our research group at INIA La Platina is advancing the integration of CRISPR-based genome editing and microRNA (miRNA) technologies to dissect and modulate key regulatory pathways in woody fruit crops. By combining bioinformatic prediction tools with experimental validation, we target genes involved in flowering, dormancy, and stress responses—traits critical for climate-resilient agriculture. Using platforms such as CRISPETA and our custom genome browsers, we design guide RNAs and evaluate off-target risks, while artificial miRNAs (amiRNAs) enable precise post-transcriptional silencing of genes like TFL1 in sweet cherry. This dual approach enhances our capacity to generate functional insights and develop novel strategies for trait improvement in species with limited transformation resources.
​
​​
WHAT WE DO
​
🧬 miRNA and CRISPR Technologies for Precision Improvement
Our group at INIA La Platina develops and integrates RNA interference (RNAi), artificial microRNAs (amiRNAs), and CRISPR-Cas systems to enhance resilience and precision in fruit tree biotechnology. We design silencing constructs and genome editing vectors targeting key regulatory genes—such as TFL1, FT, DEL1, and DST—in species like sweet cherry, grapevine, and potato. These tools allow us to modulate flowering, dormancy, pathogen resistance, and stress tolerance. Using platforms like CRISPETA, JBrowse, and our custom CRISPR Search and amiRNA Designer, we evaluate guide RNAs, off-targets, and gene architecture to support both basic research and applied breeding. Our toolkit is continuously refined to support collaborative innovation and expand the genomic capabilities of woody crops.
​
